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999
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北京泽平
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EA
货号:T_7011195-32
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英文名称:BROMINE WATER, SAT, 1 LITER
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文献和实验相关专题 Northern Blotting技术专题 Prepare glass plates for gel: Rinse plates with cold water, then clean with dishwashing liquid and warm water. Do not scratch plates. Rinse with hot tap water until all soap is removed
(Sigma G-4505) 50 ml 1 M Tris-HCl, pH 7.6 40 ml 0.5 M EDTA, pH 8.0 ddH2 O to 1 liter GET/lysozyme solution : (50 mM glucose, 25 mM Tris-HCl, pH 8.0, and 10 mM EDTA, pH 8.0 in double distilled water) 0.9 g d-glucose 2.5 ml 1 M Tris-HCl, pH
that will be over the lower left corner of the gel to later orient the blot. Nytran is preferred over nitrocellulose because it doesn''t tear as readily. Wet the 3MM in a dish of 20X SSPE. Nytran is best wetted first in distilled water, and then transferred to 20X SSPE. Place
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