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文献和实验Procedures for In Vitro Production of Bovine Embryos
with OMM + supplement microdrops (pre-equilibrated) Bench paper to cover surface 25 ml pipet automatic pipettor Mesh or styrofoam on counter X-plate Integrid petri-dish100 μm Falcon Cell Strainer50-ml Centrifuge Tubes, self standing with plug seal cap
Screening lambda cDNA or genomic libraries
at the bottom (chloroform keeps bacteria from growing but won't harm the phage).For very long term storage,add 10% DMSO,freeze in liquid nitrogen,store at -70°.Titers will range from 106-1011 phage per ml.Often a 1000-fold diluted stock of the original library is titered
Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
RSP-1T 1 1" diameter post, 1" height Newport PS-1 1 1" diameter post
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