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文献和实验Intraspinal Transplantation of Mouse and Human Neural Precursor Cells
the preparation and transplantation of human neural precursor cells (hNPCs) and mouse neural precursor cells (mNPCs) into the thoracic region of the mouse spinal cord. The techniques in this unit also describe how to prepare the mouse for surgery by performing
Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional Profiling
) 20% (v/v) fetal bovine serum (FBS) in DMEM/F12 medium (chilled to 4°C) Alexa Fluor 488 goat anti–mouse IgG2a (2 mg/ml; Invitrogen, cat. no. A21131
Wholemount staining of embryos
5'' washes with TBS. Use haematology mixer (these are really good mixers Fisher cat#14-060-1) Block protein binding sites by incubating embryos in TBS plus 2 mg/ml BSA plus o.1 (v/v) triton-X-100 plus 20 heat inactivated normal serum (donkey or goat
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