赛默飞世尔Thermo Fisher X500 Lid st
erile polythene 25 packs of 20 (pack of 500) 货号：T_70310520903- ￥1200 - 24000
- Thermo Fisher
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- T_70310520903
- 2025年07月15日
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- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70310520903
中文名称：
英文名称：X500 Lid sterile polythene 25 packs of 20 (pack of 500)
货期：热销品现货，其他请咨询
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文献和实验Recovering Plasmids from your Stab Culture
-term storage is unknown. Most labs store bacteria in 15-25% glycerol. As an example, a 25% glycerol stock can be made by adding 500 μL of an overnight culture to 500 μL of 50% glycerol in a 2 mL screw top tube. Freeze the glycerol stock tube at -80o C
2O and autoclave the columns for re-use. Phage Lysis and DNA Concentration: 1. Add 10 µl of 0.1 mg/ml proteinase K (stock solution is 20 mg/ml stored at -20 degrees C) and 25 µ l of 10% SDS to the phage fraction from the column. Mix and let it sit at room
2 O (Sigma C-7254) ddH2 O to 100 ml 10x Kinase buffer : 500 mM Tris-HCl, pH 7.6, 100 mM MgCl2, and 100 mM DTT in sterile double distilled water. 5 ml 1 M Tris-HCl, pH 7.6 1 ml 1 M MgCl2 1 ml 1 M DTT sddH2 O to 10 ml (store in 25 ml aliquots at -20
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