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文献和实验,swirl to mix. 2.Aspirate medium 3.wash plate with 10 ml cold PBS x 2.Aspirate PBS completely after the second wash. 4.add 500μl of cold PBS+ protease inhibitors and scrape cells,collect in a 1.5 ml centrifuge tube.At this point you should pool
Green lab protocol for vacuum infiltration transformation of Arabidopsis
, inoculate a 500 ml culture of YEP medium containing 50 mg/l rifampicin, 25 mg/l gentamycin and the appropriate antibiotic for your construct with a 1 ml overnight starter culture. Be sure to water your plants well the day before infiltration
Green lab protocol for vacuum infiltration transfo
to transform, inoculate a 500 ml culture of YEP medium containing 50 mg/l rifampicin, 25 mg/l gentamycin and the appropriate antibiotic for your construct with a 1 ml overnight starter culture. Be sure to water your plants well the day
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