赛默飞世尔Thermo Fisher GRINDING BA

PCR amplification of variable regions (SOE-Protocol)

. For each SOE-PCR, mix the following components: x µl VH G1 x µl VH G2 x µl VK x µl Vlambda 3 µl MgCl2 5 µl PCR buffer 4 µl 2,5 mM dNTP 0,2 µl Taq polymerase x µl H20 50 µl Total volume Add 50 µl mineral oil and run the following program

常用缓冲液配制－2

into several small screw-cap jars (~50 ml capacity) Store at room temperature  20 x Energy Regeneration System* 150 mM creatine phosphate (Boehringer #127574) 2 ml of 100 mM MgATP Stock (20 mM ATP, 20 mM MgSO4 ) 40ml 0.5 M EGTA (2 mM EGTA)

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

; Fisher BP333) T4 DNA ligase (400 U/µL) TE buffer (pH 8.0) Dilute 10X stock to 1X before use. Triton X-100 (20% v/v; VWR) Trypsin (1X; Invitrogen 25300) Equipment Aluminum foil Cell scraper Centrifuge