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文献和实验Plant DNA methylation analysis by bisulfite genomic sequencing
(see comment 3); Centrifuge Sigma 1-15K (or equivalent) >15 minutes at 4°C 12000rpm (12000 X g); Discard supernatant, add 1.0ml 70% ethanol (room temperature), vortex; Centrifuge Sigma 1-15K (or equivalent) >15 minutes at 4°C 12000rpm
Characterization of Corticotropin‐Releasing Factor (CRF) Receptors
) Vacuum aspirator: aspiration flask connected to tubing with Pasteur pipet/pipet tip (for radioactive waste) Microcentrifuge tube cutter (Fisher) or dog nail clippers
running matrix: To make approximately 200 ml, pour 200 ml of 10 mM TEAB into an Erlenmeyer flask. Add approximately 50 g of Sephadex G-50 and swirl to absorb the liquid. Suspension will settle. Store at 4°C. Prior to use, apply vacuum pressure to the flask
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