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- 文献和实验
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- 库存:
999
- 供应商:
北京泽平
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- 保修期:
1年
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EA
货号:T_70311777981
中文名称:
英文名称:X2 CULTURE FLASK 1800ML FERNBACH
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文献和实验Establishment of a Primary Culture
in MEM at 1000 rpm for 10 minutes in a standard clinical centrifuge. Remove the supernatant and resuspend the pellet in 25 ml of fresh MEM + 10% FCS. Remove 0.1 ml of the culture and determine cell concentration and viability
, you can either discard or transfer to a labeled flask and save as an additional back-up culture. F . If there is no adherence or the sample was set late in the day, incubate overnight. The next day, flood the coverslips with 1.0ml of fresh media and check
Establishment of a Primary Culture
. Move the remaining tissues of the embryo to yet another dish and wash with PBS. Mince the embryo finely with scissors and transfer the minced tissue to a flask containing PBS. Allow the tissue pieces to settle. Remove the PBS with a sterile pipette and add 25 ml
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