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麦格生物
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文献和实验Density sensitive - die if too dense or too dilute III. MAINTENANCE Split one flask of cells (most recent date) into two new T-75 flasks (VWR# BD353136) when culture is confluent. Keep other flask as a backup. Monitor growth status by microscopy
How do you culture your Drosophila cells
75 flasks (VWR# BD353136) when culture is confluent. Keep other flask as a backup. Monitor growth status by microscopy before splitting and decide whether to adjust recommended dilution factor accordingly. A. Semi-adherent cell lines - will stick
(1.05 kg/cm2 ) on liquid cycle.) SOB agar plates containing 20 mM MgSO4 and the appropriate antibiotic Standard SOB contains 10 mM MgSO4 . SOB medium, for growth of culture to be transformed Prepare
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