赛默飞世尔Thermo Fisher X960 Tubes
TrakMates 2D barcoded storage V bottom, sterile polypropylene latch rack 0.5mL(pack of 货号：T_70310111443- ￥1200 - 24000
- Thermo Fisher
- 美国/芬兰/中国/韩国/墨西哥
- T_70310111443
- 2025年06月30日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70310111443
中文名称：
英文名称：X960 Tubes TrakMates 2D barcoded storage V bottom, sterile polypropylene latch rack 0.5mL(pack of
货期：热销品现货，其他请咨询
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文献和实验Analysis of Proteins using Small Format 2D Gel Electrophoresis
twice with PBS-A and lysed in 0.2 ml of 2D lysis buffer (0.01M Tris-HCl, pH 7.4, 1mM EDTA, 8M urea, 0.05M dithiothreitol, 10% (v/v) glycerol, 5% (v/v) Nonidet P-40, 200µg/ml RNAse A, 6% (w/v) pH 3.5-10 carrier ampholytes ("Resolyte", Merck
Preparation of Bacterial Proteins for Analysis by 2D-PAGE
the NP40 at a concentration of 2-4% (w/v). Once prepared, the 2D Lysis Buffer is divided into smaller volumes (e.g. 1-1.5 ml) and stored frozen in sterile microfuge tubes at -70oC until required. A tube of lysis buffer is thawed as required
Analysis of Proteins using Small Format 2D Gel Electrophoresis
-A and lysed in 0.2 ml of 2D lysis buffer (0.01M Tris-HCl, pH 7.4, 1mM EDTA, 8M urea, 0.05M dithiothreitol, 10% (v/v) glycerol, 5% (v/v) Nonidet P-40, 200µg/ml RNAse A, 6% (w/v) pH 3.5-10 carrier ampholytes ("Resolyte", Merck Ltd.)) for 5 minutes on ice
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