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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2
Immuno-Laser Capture Microdissection
with spot sizes of 50 to 60 µm (C) and 15 to 20 µm (D) in diameter, as seen during LCM without coverslip. The smaller spot size allows avoidance of interspersed T cells. Magnification x100 (C) and x400 (D). Fig. E: Lymph node stained
Bigge, J.C., Patel, T.P., Bruce, J.A., Goulding, P.N., Charles, S.M., and Parekh, R.B. 1995. Nonselective and efficient fluorescent labeling of glycans using 2‐amino benzamide and anthranilic acid. Anal. Biochem. 230:229‐238
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