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999
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北京泽平
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热销品大量现货,其余请咨询
- 保修期:
1年
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EA
货号:T_701OB1020-01
中文名称:
英文名称:GOAT ANTI MOUSE IGM UNLB 1.0MG
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文献和实验Wholemount staining of embryos
5'' washes with TBS. Use haematology mixer (these are really good mixers Fisher cat#14-060-1) Block protein binding sites by incubating embryos in TBS plus 2 mg/ml BSA plus o.1 (v/v) triton-X-100 plus 20 heat inactivated normal serum (donkey or goat
Double immunohistochemistry for BCL6 and PNA for Germinal Center detection.
the slides without washing and apply biotin-conjugated PNA, in a moist chamber, at RT overnight (minimum for 2 hr.). Biotin-conjugated PNA (Sigma, StLouis, MO, use at 1.0 µg/ml) 5- Wash twice in TBS-T. 6- add the biotin-conjugated goat anti PNA antibody
Double immunohistochemistry for BCL6 and PNA for Germinal Center detection
, in a moist chamber, at RT overnight (minimum for 2 hr.). Biotin-conjugated PNA (Sigma, StLouis, MO, use at 1.0 µg/ml) 5- Wash twice in TBS-T. 6- add the biotin-conjugated goat anti PNA antibody (100 to 200 µl; 1:400) and incubate for 45 min
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