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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_7011590-1
中文名称:
英文名称:BUFFER, RICCA, PH 9.00, 4 L
货期:热销品现货,其他请咨询




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文献和实验Southern Hybridization Protocols
-phosphate buffer pH 7.2 7% (w/v) SDS 2% (w/v) BSA 1 mM EDTA pH 8.0 Preparation of solution (not easy to prepare therefore follow the recipe): Prepare 0.3 M Sodium-phosphate buffer by adding 40.2g NaH2PO4 to 400 ml sterile distilled water
7.8 or Binding Buffer.(6) BD tubing 14-170-12F (Fisher).(7) Polyethylene tubing Clay Adanes.(8) 18 Gauge needles.(9) Binding Buffer (BioRad): make 1L. 314g/L ddH2O and filter through 0.22 um, filter. pH=9.0.(10) Elution buffer (BioRad): make 500 ml. 11g/500 ml
X denaturing buffer : 200 mM Tris-HCl, pH 9.5, 1 mM EDTA, 10 mM spermidine in double distilled water. 2 ml 1 M Tris-HCl, pH 9.5 20 μl 0.5 M EDTA, pH 8.0 1 ml 100 mM spermidine ddH2 O to 10 ml (aliquot and store at -20℃) Diatomaceous earth (100 mg/ml
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