赛默飞世尔Thermo Fisher ACETIC ACID

Biotinylation of a Propargylated Cyclic (3′‐5′) Diguanylic Acid and of Its Mono‐6‐Thioated Analog Under “Click” Conditions

) Chloroform (CHCl 3 , Fisher) Acetic acid (AcOH, Acros) Silica gel (60 Å, 230 to 400 mesh, Merck

Derivatization of Free Natural Glycans for Incorporation onto Glycan Arrays: Derivatizing Glycans on the Microscale for Microarray and Other Applicati

7:3 (v/v) dimethyl sulfoxide (DMSO), ACS grade (Fisher Scientific)/acetic acid Sodium cyanoborohydride 2‐(N ‐aminoethyl

SDS-PAGE

staining your gel. The stain is: 1 g Coomassie brilliant blue R-250 50 ml glacial acetic acid 500 ml MeOH 450 ml H20 (Note: This is a somewhat weak stain recipe: it destains faster and gives a prettier gel, but may be less sensitive