赛默飞世尔Thermo Fisher SPACERS 2CM

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

; Fisher BP333) T4 DNA ligase (400 U/µL) TE buffer (pH 8.0) Dilute 10X stock to 1X before use. Triton X-100 (20% v/v; VWR) Trypsin (1X; Invitrogen 25300) Equipment Aluminum foil Cell scraper Centrifuge

SDS-PAGE

1. To use commercial BRL boxes: Clean the plates with soap and hot water, wipe dry, scrub with ethanol, wipe dry, and clamp them together with spacers in between. The notched spacers interlock to form a tight seal. I use the thin (0.8 mm) spacers

Detection and Analysis of Protein‐Protein Interactions of Organellar and Prokaryotic Proteomes by Blue Native and Colorless Native Gel Electrophoresis

.1 .  J The recipes produce 34 ml of each separating gel solution and 20 ml of sample gel, which are adequate for one preparative gel with 3‐mm × 16‐cm × 16‐cm dimensions (Bio‐Rad). When using other spacers or different