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货号:T_7016584-16
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英文名称:PYRIDINE-BARBITURIC RGT,500ML
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
for the restriction enzyme to be used in Step 16). 13. Pellet the cells by centrifugation. Resuspend in 500 µL of 1.14X restriction enzyme buffer. Transfer to 1.5-mL tubes. 14. Add 7.5 µL of 20% SDS (to a final concentration
2 CO 3 ,Fisher) Tetrahydrofuran (THF; Acros) Pyridine, anhydrous (Acros)
Double immunohistochemistry for BCL6 and PNA for Germinal Center detection.
antibody, in a moist chamber, at RT overnight (minimum for 2 hr.). Rabbit anti BCL-6 (Santa Cruz Biotechnology, Santa Cruz, CA: Bcl-6 (N-3): sc-858, use at 0.5 µg/ml) 16- Wash twice in TBS-T. 17- add the AlkPhos-conjugated secondary antibody (100 to 200
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