赛默飞世尔Thermo Fisher Nalgene 低温冻

Procedures for In Vitro Production of Bovine Embryos

(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15

细胞培养完整手册

) 3.步骤： 3.1.冷冻前一日前更换半量或全量培养基，观察细胞生长情形。 3.2.配制冷冻保存溶液(使用前配制)：将DMSO 加入新鲜培养基中，最后浓度为5-10 ％，混合均匀，置于室温下待用。 3.3.依细胞继代培养之操作，收集培养之细胞，取少量细胞悬浮液(约0.1 ml) 计数细胞浓度及冻前存活率。 3.4.离心，去除上清液，加入适量冷冻保存溶液，使细胞浓度为1-5 x 106 cells/ml，混合均匀，分装于已标示完全之冷冻保存管中，1 ml/vial，并取少量细胞悬浮液作污染

Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum

with deionized water, and mix Mouse osteocalcin standard, one vial, 200 ng, lyophilized: reconstitute standard with exactly 2.0 ml of sample buffer (yields a 100 ng/ml solution); use