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文献和实验V294.PART II,Chapter 2 Boyden法体外趋化试验
of ethylene diamine tetraacetic acid (0.53 mM) and 0.01 g of phenolred in 1 L of phosphate-buffered saline, pH 7.4. 7. 2.5% (w/v) Trypsin (cat. no.15090-046; Gibco Invitrogen): dilute 1:25 in Versenebefore use. 8. Collagen from calf skin (cat. no. C
v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性
volume withdistilled water. 6. Destaining solution: 30% (v/v) methanol and 10% (v/v) acetic acid. 7. Gel drying solution: 50% (v/v) methanol and 10% (v/v) acetic acid. 8. Cellophane wrap. 2.5. Immunoblotting 1. Mini-gel transfer apparatus to match
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
phosphate-buffered saline (PBS) containing 0.1% (w/v)bovine serum albumin (BSA) at a concentration of 50 μg/mLand stored in aliquots at –20°C (see Note 2 ). 3. Mouse L cells that express and secrete Wnt3a (ATCC, Manassas,VA; #CRL-2647) and the control L
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