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- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70109-841-267
中文名称:
英文名称:MEMBRANE CAP GL32
货期:热销品现货,其他请咨询








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文献和实验Northern Blotting方法(Howell Lab)
Fixing Bake membrane in 80℃ vacuum oven for 20 min. UV crosslink (on optimal setting of Fisher Crosslinker) the membrane (face side up). Store between 2 pieces of chromatography paper in a plastic bag. Labelling the probe
, 28304) Prime-It II Random Primer Labeling Kit (Stratagene, 300385) [alpha-32P]dCTP (ICN) Salmon Sperm DNA (Stratagene, 201190) SDS solution, 20% w/v (Bio-Rad, 161-0418) Zeta-Probe Genomic Tested Blotting Membrane (nylon) (Bio-Rad, 162-0196) Gel Blot
Phosphoamino acid analysis:Mark Kamps's method
will work! Keep the membrane wet and wrap in Saran wrap. Add radioactive markings for orientation of filmlater Expose to film. 2.Cut out band of interest,re-wet in methanol,rinse well in water and place in a screw-cap tube containing 150 µl 5.7 NHCl
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