赛默飞世尔Thermo Fisher 枪头 EPPENDOR

In Situ HYBRIDIZATION TO TISSUE SECTIONS

/sample to 10ml formamide, boil 1', chill and add 2.5ml 5X loading buffer (0.25% BPB, 0.35% XC, 15% Ficoll in 5X TBE) - End - labeled DNA fragments (e.g., pBR322/Hinf I) may be used as size markers - Run 200V, ‰ 4h (BPB about 2/3 down the gel) - Soak 20

Stripping Western Blots

, put in an eppendorf, rock 10-20’ at 4C and then spin 10’. Save supernatent―determine protein concentration 4. Immunoprecipitate 100 μg of protein (if you use K12 antibody, and 200 μg of protein if you use AB-3 antibody) in a total of 800 μl. Use 1 μg