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货号：T_70311834602
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英文名称：X12 VOLUMETRIC PIPETTE 1,5ML CL A
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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
volume of average-sized sample. Add 0.5 vol. 8 M LiCl, mix and stand at 4°C overnight. 10. Spin at 12000g, 4°C, for 15 min in a fixed angle rotor. Thoroughly resuspend pellet in 4 ml sterile gd (glass distilled) H2 O with pasteur pipette. 11. Extract twice
Procedures for In Vitro Production of Bovine Embryos
(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15
Clone Genes From a Phage Library
, you need to use a seal-a-meal bag, with about 1.5 to 2 ml hybe solution per small plate-sized filter. (I use 20 ml for 12 filters) • Put to 65 degrees for 3 hours to overnight or longer, with sufficient agitation to expose all the filters
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