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- 库存:
999
- 供应商:
北京泽平
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热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70103-500-727
中文名称:
英文名称:MINI-PLUS COMB 1MM X 20MC WELL
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文献和实验., then load gel (15-25µl for Hoefer mini-gel, 20-50µl for midi-gel). 4. Run until BPB-dye reaches bottom of gel (about 1-1.5hr for mini-gel at 40mA, about 2-2.5 hours for midi-gel at 200V). 5. If staining protein, cut away stacking gel and place running gel
Basic procedures for bacteria culture
microcentrifuge tube, invert to mix, and incubate in an ice-water bath for at least 10 minutes. It is possible to place the sample at -20degC overnight at this stage. 2. Centrifuge at 12,000 rpm in a microcentrifuge (Fisher) for 15 minutes at 4 degC, decant
20 solution (PBS-T). This was repeated 6 times and the final pellet was stored at -20°C.2.4 SDS-Polyacrylamide Gel Electrophoresis2.4.1 Sample preparationEach sample consisting either of endothelial lysate (10mg protein) or immunoprecipitate (prepared
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