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货号:T_70122-282148
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英文名称:SAF-DE-CAP TUBE DECAPER 200/CS
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文献和实验Procedures for In Vitro Production of Bovine Embryos
from the bottom of the Percoll gradient.5. Place the sperm pellet into a 15 ml conical tube containing 10 ml Sp-TALP and place in a warm centrifuge carrier before centrifuging for 5 min at 200 x g. The exact speed is probably not critical - do a low-speed centrifugation
RNA purification using TRizol--TRIZOL法提取RNA
of chloroform to each tube.b.Cap each tube. Shake samples vigorously by hand for 15 seconds.c.Incubate samples for 5 minutes at room temperature.d.Centrifuge samples for 15 minutes at 12,000 x g at 4oC.3. RNA Precipitation a.Transfer the upper aqueous phase
Northern Blotting方法(Howell Lab)
inverted because the RNA pellets on the bottom. (DNA can be recovered from the CsCl layer.) Cut off the tube bottoms (keep inverted) and resuspend the pellet in 100 µl 0.3 M NaOAc (pH 6) buffer. Transfer to a screw-cap 1.5 ml Eppi. Wash tube
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