赛默飞世尔Thermo Fisher BROMATE-BRO

基于PCR技术的染色质沉淀分析

. Transfer 5 µl of the PCR product to another PCR tube. Bring the volume to 25 µl with a newly prepared PCR mix containing [{alpha}-32P]-dCTP (10 µCi/µl, specific activity 3000 Ci/mmol) and fresh dNTPs. The amount of [{alpha}-32P]dCTP

【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离

) High-throughput retroviral tagging to identify components of specific signaling pathways in cancer. Nat Genet 32, 153–9. 7. Suzuki, T., Minehata, K., Akagi, K., Jenkins,N. A. and Copeland, N. G. (2006) Tumor suppressor gene identification using

Cloning via Restriction Digests

instructions. Example: a. Combine the following in a microfuge tube (10 μL total volume): 1 μL Vector DNA 3 μL Insert DNA 1 μL 10x Ligase Buffer 1 μL T4 DNA Ligase 4 μL H2 0 (to bring total volume to 10 μL) Tip