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文献和实验Protocol for Trichloroacetic Acid (TCA) Precipitation
and Equipment Required Borosilicate tubes, 12 x 75 mm (e.g., Fisher Cat #14-961-26) 10% TCA (e.g., Acros Cat # 42145-5000; make a 10% (w/v) solution in water) Warning: TCA is caustic! Carrier nucleic acid, 1 mg/ml (e.g., sheared fish
Measurement of Arginine Metabolites: Regulators of Nitric Oxide Metabolism
) L‐arginine, L‐citrulline, proline, L‐ornithine, ADMA, SDMA, and NMMA 0.1 M HCl (ACS reagent grade; Fisher)
Measurement of the Nitric Oxide Synthase Activity Using the Citrulline Assay
) by the oxidation of the amino acid L -arginine. As a dissolved gas, NO is an unusual neurotransmitter. It is not packaged in synaptic vesicles and released by exocytosis upon membrane depolarization, but rather diffuses from its site
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