赛默飞世尔Thermo Fisher Test tube f

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

; Fisher BP333) T4 DNA ligase (400 U/µL) TE buffer (pH 8.0) Dilute 10X stock to 1X before use. Triton X-100 (20% v/v; VWR) Trypsin (1X; Invitrogen 25300) Equipment Aluminum foil Cell scraper Centrifuge

A quick RNA mini-prep for Neurospora mycelial cultures

is as follows: 1. Conidia from slants (grown in 16 x 150 mm test tubes containing 8 ml of solid medium) are resuspended in 50 ml of Horowitz complete medium (Horowitz 1947 J. Biol. Chem. 171:255-262) and the cultures grown overnight with shaking at 30°C

Purification of mAb (IgG)

. Test each tube w/H2O and discard.Add protein mixture to dialysis tube. Stir slowly in 100 mM Tris buffer or Binding buffer until pink color is out.Transfer protein mixture (Ab) to 50 ml Falcon tubes to determine volume.Dilute the mixture