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英文名称:X960 0,1-10µL LG TIP RACK PST
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文献和实验Procedures for In Vitro Production of Bovine Embryos
Needed 2 x 1 L sterile saline (transport saline) 1 aliquot of stock 16 (Pen-Strep 10 ml) per bottle of saline 3 to 4 bottles of oocyte collection medium (OCM) (400 ml) 1 aliquot of stock 11 (glutamine 4 ml) per bottle of OCM
In Situ HYBRIDIZATION TO TISSUE SECTIONS
. The devittellinized embryos will sink to the bottom. Transfer the embryos to a vial with a wide bore pipet tip. Wash 3X with 90% MeOH/EGTA. Rehydrate embryos (0-2 and 1-3 hr embryos need not be rehydrated through paraformaldehyde, just leave
Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering
in 20 mL of DMEM to be counted using a hemocytometer, and place the cells on ice. 12) Place 50 μl of the cells into a Trypan Blue solution (75 μl Trypan Blue, 125 μl PBS), mix well before placing 10 μl in the hemocytometer
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