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999
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北京泽平
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货号：T_701OB6140-01
中文名称：
英文名称：RBT ANTI HUMAN IGG UNL 1.0MG
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文献和实验Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional Profiling
) 20% (v/v) fetal bovine serum (FBS) in DMEM/F12 medium (chilled to 4°C) Alexa Fluor 488 goat anti–mouse IgG2a (2 mg/ml; Invitrogen, cat. no. A21131
Transplantation Models to Characterize the Mechanisms of Stem Cell–Induced Islet Regeneration
(DPBS, no Ca2+ no Mg2+ ; Life Technologies, cat. no. 14190250) containing 5% (v/v) fetal bovine serum (FBS) Desired fluorescently labeled anti‐human antibodies and isotype controls (Table
), GSK-3b (dilution1:1,000; 1 h at RT), or b-actin (dilution 1:10,000; 1 h at RT). 5. Wash the membrane three times for 5 min each in TBS-T. 6. Incubate the membrane for 1 h at RT with 30 mL of TBS-Tcontaining anti-mouse or anti-rabbit IgG
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