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文献和实验V294.PART II，Chapter 2 Boyden法体外趋化试验
of an inhibitor that is specificfor an intracellular signaling molecule or a functional blocking antibody that isspecific to a cell surface protein on cell motility by adding it to the upper chamber,in which cells are loaded. Actually, this type of experiment
QUALITATIVE ANALYSIS OF DNA FRAGMENTATION BY AGAROSE GEL ELECTROPHORESIS
at 20,000xg for 10 min at 4°C. 6. Carefully transfer supernatants in new tubes labeled T (top). 7. Add to the small pellet in tubes B 0.5 ml of TTE solution. 8. Add to the 0.5 ml volume present in tubes B, S and T, 0.1 ml of ice-cold 5M NaCl
In Situ HYBRIDIZATION TO TISSUE SECTIONS
. Giemsa (SIGMA GS-500): Use at a 1:20 dilution in 10mM NaHPO4, pH 6.8. 2mg/ml Glycine (SIGMA G7126) in 1X PBS 0.2N HCl 2M HEPES (SIGMA H3375, FW 238.3) Heptane Immersion Oil (Fisher 12-370A, Cargille Type A) Kodak
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