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- T_70311972638
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70311972638
中文名称：
英文名称：X1000 COVER SLIP 24X32 MM N°1,5
货期：热销品现货，其他请咨询
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文献和实验rpm at 4°C Prepare fluorochrome solution (see above) Cover each slide with 125 µl of the fluorochrome solution and a 24x60 mm2 cover slip. Incubate 20 min in a moist chamber at 37°C. Remove cover slips and wash slides 3 x 3 min at 45°
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
sec. break x 3 times, 20% amplitude with a small tip). Now the length of your chromatin DNA should be 200-1000 bp. (13) Centrifuge for 20 min at 14000 rpm, 4°C. Recover supernatant. This is the soluble sheared chromatin fraction. This can be stored
The ribonuclease protection assay (RPA)
plate, and absorb the gel to filter paper. Cover the gel with Saran wrap and layer between two additional pieces of filter paper. Place in the gel dryer vacuum for ~ 1 hour at 80°C. Place the dried gel on film (Kodak X-AR) in a cassette
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