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货号：T_70103570C
中文名称：柔性手柄烧瓶刷
英文名称：BRUSH FOR 1000ML FLASK
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文献和实验Monoclonal Antibody Production Protocol
the PEG. Do not disturb the cells. Spin at 1000 rpm for 5 min. Aspirate medium, resuspend cells by tapping. Slowly add 5 ml of 37°C IMDM-m. Bring to 20 ml and add to the flask in the incubator. (If using feeder cells, add them at this point; 106 cells
Large-scale yeast transformation
. Resuspend yeast in the 90 mls LiAc/TE, put back in same flask (rinsed with sterile water). Shake at 30o C for 30 mins. Add 600 ul beta-mercaptoethanol. Shake at 30o C for 30 minutes. Add 1.7ml of 10mg/ml ss carrier DNA 3 . Cells ready
. After adding , swirl the tube gently to mix and dilute the PEG. Do not disturb the cells. Spin at 1000 rpm for 5 min. Aspirate medium, resuspend cells by tapping. Slowly add 5 ml of 37°C IMDM-m. Bring to 20 ml and add to the flask
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