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文献和实验AMNIOTIC FLUID CULTURES ON COVERSLIPS
ml conical centrifuge tubes. If the sample is received differently, transfer to two tubes. Centrifuge the tubes for 6-8 minutes at 150 x g (900 rpm). Leaving 0.5 ml, transfer supernatant to a 15 ml centrifuge tube (this will be frozen for back-up AFP
the tube after removing a filament. We use a Kopf needle/pipette puller Model 750, from David Kopf Instruments, Tujunga, CA. Turn the machine on (switch at back right). Push the button in the back to reset the programs and get "0000" displayed. Flick
for the tissue to adhere to the surface; then add 1ml fresh media. The p-dish will be your back-up if the coverslips don't grow or get contaminated. D . Allow the tissue in the centrifuge tube to settle while you prepare and sterile filter the collagenase
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