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货号：T_70118-295A
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英文名称：SOFT-STEP MAT 2X3 BLACK
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文献和实验Procedures for In Vitro Production of Bovine Embryos
(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15
Transfection of Fibroblasts - SuperFect 1) Seed cells in mid- to late afternoon and let grow overnight. NIH3T3 2x105/well of 6-well dish 6x105/6cm plate REF52 1x105/well of 6-well dish 2.5x105/6cm plate WI
In Situ HYBRIDIZATION TO TISSUE SECTIONS
mixing. Incubate 10' during which time the stirring may be slowed down. -If gelatin slides are used, acetylation is unecessary and step 19 may be omitted. Rinse in 1X PBS, 2X 3'. Dehydrate through 30%, 60%, 80%, 95% and 100% EtOH
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