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999
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北京泽平
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1年
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EA
货号:T_70313524680
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英文名称:Cover for use with MS-53M stirrer MEDLINE
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文献和实验PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
Tert-amyl alcohol 5.0 ml (Fisher: A-730-1). Dissolve tribromethanol in Tert-amyl alcohol, then add to 200 ml distilled water. Place on a magnetic stirrer until solution is in one phase. Store in brown bottle and keep refrigerated until use
Noninvasive Human Nuclear Transfer with Embryonic Stem Cells
. MATERIALS Reagents Calcium ionophore (10 µM) (Sigma-Aldrich) Culture media (Cooper Surgical) Use HEPES media to wash oocytes whenever media are changed, for example between cytochalasin B and cleavage medium (Steps 9 and 10
PRODUCTION OF ES CELL CHIMERAS BY AGGREGATION WITH EIGHT-CELL STAGE EMBRYOS
, then add to 200 ml distilled water. Place on a magnetic stirrer until solution is in one phase. Store in brown bottle and keep refrigerated until use. Should be warmed and shaken before use. Dosage is 0.2 ml/10 g body weight . Method:
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