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EA
货号：T_7011050-16
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英文名称：BORAX, 1% AQUEOUS, 500 ML
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
for the restriction enzyme to be used in Step 16). 13. Pellet the cells by centrifugation. Resuspend in 500 µL of 1.14X restriction enzyme buffer. Transfer to 1.5-mL tubes. 14. Add 7.5 µL of 20% SDS (to a final concentration
Preparation of End-Labeled DNA Probes by Conventional Kinase for DNA Footprinting Analysis
of approximately 500 μg/ml. 11. Store at -20°C. C. Kinasing Reaction 1. Combine the following: 20 μl of CIP-treated DNA (10 μg, from Step #B11) 4 μl of 10X Forward Kinase Buffer 14 μl of ddH2 O
RNA purification using TRizol--TRIZOL法提取RNA
Reagents and EquipmentsTRIzol Reagent (Life Technologies cat# 15596-026) or TRI reagent (Sigma cat # T-9424)DEPC (RNase free) water or 0.5% SDS solution in DEPC treated waterChloroform (Fisher )Isopropyl alcohol (2-Propanol) (Fisher)75% Ethanol
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