赛默飞世尔Thermo Fisher BORAX, 1% A

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

for the restriction enzyme to be used in Step 16). 13. Pellet the cells by centrifugation. Resuspend in 500 µL of 1.14X restriction enzyme buffer. Transfer to 1.5-mL tubes. 14. Add 7.5 µL of 20% SDS (to a final concentration

Preparation of End-Labeled DNA Probes by Conventional Kinase for DNA Footprinting Analysis

of approximately 500 μg/ml. 11. Store at -20°C. C. Kinasing Reaction 1. Combine the following: 20 μl of CIP-treated DNA (10 μg, from Step #B11) 4 μl of 10X Forward Kinase Buffer 14 μl of ddH2 O

RNA purification using TRizol--TRIZOL法提取RNA

Reagents and EquipmentsTRIzol Reagent (Life Technologies cat# 15596-026) or TRI reagent (Sigma cat # T-9424)DEPC (RNase free) water or 0.5% SDS solution in DEPC treated waterChloroform (Fisher )Isopropyl alcohol (2-Propanol) (Fisher)75% Ethanol