赛默飞世尔Thermo Fisher COMB 10 SAM

SEQUENCING GELS

      10X TBE, per liter      Sequencing dye       -------------------     ----------------        108g Tris base          80% formamide        55g boric acid          10mM NaOH        40ml 0.5M EDTA           1mM EDTA             

SDS-PAGE

for routine analytical gels, and 1.5 mm spacers to run more sample. I usually use a comb that makes 20 wells.2. Solutions:I. 1.5 M Tris-HCl, pH 8.8II. 1 M Tris-HCl, pH 6.8III. 30% acrylamide/bisacrylamide (37.5:1). This solution can be bought commercially

Agarose Gel Electrophoresis for the Separation of DNA Fragments

Sigma E7637 Carcinogenic—needs to be disposed of as hazardous waste Glacial acetic acid Fisher BP2401