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文献和实验Staining protocols for placental explant cultures
. Orient it as during culture. Encase the gel fragment in aqueous hardening mountant (Histotec, Serotec, UK). Do not use a cover slip. Incubate at room temperature overnight in the dark. The culture can now be inspected directly using water or oil
Staining protocols for placental explant cultures
Encase the gel fragment in aqueous hardening mountant (Histotec, Serotec, UK). Do not use a cover slip. Incubate at room temperature overnight in the dark. The culture can now be inspected directly using water or oil immersion objectives
SILVER STAINING OF NUCLEOLAR ORGANIZING REGIONS (NOR)
colloid solution. With a fresh pipet add 11 drops of silver nitrate working solution. Working quickly, mix by pipeting up all liquid and then place 4 drops of mixture on the slide. Cover with a 24 x 60 mm coverslip and blot between paper towels to remove
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