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文献和实验Preparation of Mitochondria from Rat Liver
, and 1 mM EDTA, pH 7.2 for homogenizing. We use a shearing type homogenizer called 'Tissuemizer,' with T25 stainless steel shaft, made by Tekmar, Inc. (Cincinnatti, Oh). A teflon-on-glass (Potter-Elvehjem) homogenizer can also be used, but it takes longer
called 'Tissuemizer,' with T25 stainless steel shaft, made by Tekmar, Inc. (Cincinnatti, Oh). A teflon-on-glass (Potter-Elvehjem) homogenizer can also be used, but it takes longer. The Polytron is fast but prolonged or overly vigorous homogenization can be damaging
Preparing Mitochondria from Rat Liver
medium to tissue is important, as is the depth of liquid in the container when using a shearing type homogenizer. The specific instrument we use is a "Tissuemizer" with T25 stainless steel shaft (Tekmar, Inc., Cincinnatti, OH). A teflon-on-glass (Potter
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