赛默飞世尔Thermo Fisher pH 4 500ml缓

Purification of Plasmid from 50 ml-culture

4. Resuspend the pellet in 5 ml of Solution I. 5. Add 10 ml of Solution II. Mix well by inverting the bottle more than 10 times. The solution should be clear after mixing Solution II. 6. Add 7.5 ml of Solution III. Mix well as above. 7. Spin

BAC DNA Isolation from 200 ml Cultures by a Cleared Lysate Method

by centrifugation at 5K rpm for 15 minutes in a 250 ml bottle in the RC5-B using the GSA rotor, cell pellets should be frozen and stored at -70 degrees C. 2、Prior to use, the cells from 200 ml growth were thawed and resuspended in 8 ml of just 10 mM of EDTA, pH

CCMB buffer to make chemical competent cell

does not appear to be necessary Test competence (see below) Good cells should yield around 100 - 400 colonies 4. CCMB80 buffer 10 mM KOAc pH 7.0 (10 ml of a 1M stock/l) 80 mM CaCl2.2H2O (11.8 g/l) 20 mM MnCl2.4H2O (4.0 g/l) 10 mM MgCl2.6H2O (2.0 g/l) 10