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999
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北京泽平
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EA
货号:T_70312953521
中文名称:
英文名称:Ferrule for 1.8mm O.D. Ultra-Ware Standard version 3/16 O.D.
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
polymerase (REDTaq; Sigma D4309) (for polymerase chain reaction [PCR]; see Step 45 and Discussion) dNTP set (100 mM; Invitrogen) (for PCR; see Step 45 and Discussion) Ethanol (70% and 100%) Fetal bovine serum (10% in PBS) Formaldehyde
, this method for preparing competent E. coli from Inoue et al. (1990) can challenge the efficiencies achieved by Hanahan (1983). However, under standard laboratory conditions, efficiencies of 1 x 108 to 3 x 108 transformed colonies/mg of plasmid DNA
Construction and Characterization of Adenovirus Vectors
) Tubes (polystyrene, conical, 15-mL) Tubes (polystyrene, round-bottom, 5-mL) Tubes (ultracentrifuge, Quick-Seal, 16- x 76-mm) (Beckman 344322) Tubes (ultracentrifuge, 14- x 89-mm) Ultracentrifuge Vortexer Water baths
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