赛默飞世尔Thermo Fisher 1/2 oz / 15

ChIP protocol for X. laevis Lens1/FoxE3 enhancer

(9)  Sonicate the homogenate on ice (10 sec. pulse + 50 sec. break x 6 times, 20% amplitude with a small tip of Branson sonifier (Fisher Dismembranator Model 500)). This step releases chromatin DNA from cells without solubilizing yolk proteins that make

Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas

of one rat spleen (approx. 10**8 cells) together with 6 x 10**7 Y3/Ag1.2.3 per fusion (the other half of the spleen cells can be frozen as a backup in 90% FCS +10% DMS0). c) Try and keep all cells, PEG and medium components at 37 deg. C

SQ Blood DNA Maxi Protocol for 4-10 ml whole blood

15 ml microcentrifuge tubes 3. Water baths preset at 37°C 4. Paper towels 实验步骤   1. Add one volume of whole blood (or bone marrow) to a nuclease-free 50 ml centrifuge tube containing 3 volume of Buffer ERL. Mix