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北京泽平
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货号:AB-1389/1000
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英文名称:Abgene 2 ml 2D Barcoded Screw Cap Tubes, Bulk, 1000/case
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文献和实验Preparation of Sonicated Human DNA
reading of 1.0 = 50 �/ml of DNA, the concentration of the undiluted solution is 50 x 84 = 4200 �/ml, or 4.2 mg/ml. If the present volume is 100 ml (420 mg), add enough TE to adjust the final concentration to 2.5 mg/ml. (In this case, add 68 ml TE
Northern Blotting方法(Howell Lab)
buffer. Pour GITC/Cell mix into (14x95 mm) polyallomer tubes with 4 ml CsCl buffer. Balance and top off tubes with more GITC buffer. Spin tubes on the SW40Ti rotor for 20 hr at 32,000 rpm (18℃). Pour off liquid and keep tubes
RNA purification using TRizol--TRIZOL法提取RNA
microcentrifuge tubes.b.Centrifuge for 1 minute to pellet the cells.c.Pour off the supernatant.d.Add 1 ml of TRIzol or TRI reagent to the tubes.e.Lyse cells by repetitive pipetting.f.Centrifuge homogenate at 12000 x g for 10 minutes at 4℃.g.Transfer the homogenate
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