货号：358206 BD FalconPolyethyl
ene Plug Seal Screw Caps for 50mL Conical Bottom Centrifuge Tubes, Sterile, 50/Pack, 1000/Case- ￥1219.46
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- 2025年07月15日
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文献和实验Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering
with ice in the sterile culture hood. 2) Place a 250 mL beaker with 30-40 mL of Betadine into the hood. 3) Add 50 mL/litter of PBS-glucose into a bottle, seal and place into a 37°C water bath. 4) For each person
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
with large probe 1'' 80% power, freeze in lN, thaw at 37 C, sonicate again on ice, solution should become viscous. 6) add 1mg DNAse and RNAse, incubate on ice 15''. 7) spin 7.5k rpm 4 C ss34 10''. 8) transfer supernatants to conical screw caps
Biotech, Cat# 17-0618-01) on a rotator at 4 °C for 30 min. 6) Incubate precleared lysates with anti-HA antibody on ice for 2 h. 7) Add 30 μl Protein G beads and incubate on a rotator at 4 °C for 2 h. 8) Wash immunocomplex once with cold lysis buffer
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