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文献和实验Procedures for In Vitro Production of Bovine Embryos
with OMM + supplement microdrops (pre-equilibrated) Bench paper to cover surface 25 ml pipet automatic pipettor Mesh or styrofoam on counter X-plate Integrid petri-dish100 μm Falcon Cell Strainer50-ml Centrifuge Tubes, self standing with plug seal cap
coating with polymer or adding a layer of metal. Place the slide into a sterile rectangular four-chamber cell culture vessel (or use tissue culture slide flask if polystyrene surface is desired). Count cells and seed 1.5 x 106 cells onto the slide in 5 ml
" Solutions. 1. A molar solution is one in which 1 liter of solution contains the number of grams equal to its molecular weight. Ex. To make up 100 ml of a 5M NaCl solution = 58.456 (mw of NaCl) g x 5 moles x 0.1 liter = 29.29 g in 100 ml
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