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文献和实验Single and Double FISH protocols for Drosophila
); dissolve in sterile H2O, divide into 50 ul aliquots and store at -20oC) . 4. 2mg/ml glycine in PBS. 5. RNA hybridization solution: 50% formamide, 5X SSC, 100 ug/ml heparin, 100 ug/ml sonicated salmon sperm DNA and 0.1% Tween 20). Filter through a 20
Southern Hybridization Protocols
3. Leave the wash at 65℃ for 30 min. Repeat.Take out the membrane and dry it on paper towel. Wrap with Saran film & place it in a film cassette. Monitor radioactivity of the membrane to estimate exposure times: >1000 counts: 2 hours; 200-300 counts, 24
were from Fisher. Sephadex G-50 resin (Cat. No. G-50-150) for purification was from Sigma. Columns were fashioned by removing the cotton from the top of one of these pipets and using a portion of it to plug up the tip. Secure the “column” vertically to a ring stand
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