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文献和实验Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering
, 0.45 μm bottle top filters with glass pre-filters, and 0.2 μm bottle top filters with glass pre-filters. The solution is aliquoted into 1 mL and 3 mL aliquots and stored at -20°C. 2) Prepare a 25 U/mL stock solution of thrombin
Procedures for In Vitro Production of Bovine Embryos
Needed 2 x 1 L sterile saline (transport saline) 1 aliquot of stock 16 (Pen-Strep 10 ml) per bottle of saline 3 to 4 bottles of oocyte collection medium (OCM) (400 ml) 1 aliquot of stock 11 (glutamine 4 ml) per bottle of OCM
Competent cells, calcium chloride method, E. coli, description
Reference: Dagert and Ehrlich (1979) Gene 6:23-28. Grow 5 ml overnight of the strain in 2 X YT in a standard container. Screw cap tight and lie flat on the shaker for good aeration. Dilute overnight culture
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