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货号：T_70122-156-785
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文献和实验Subculture of Suspension Cell Lines
are known to adhere strongly. Pipette trypsin/EDTA (Prod. No. T4049) onto the washed cell monolayer using 1ml per 25cm2 of surface area. Rotate flask to cover the monolayer with trypsin. Decant the excess trypsin. Return flask to the incubator and leave
for the tissue to adhere to the surface; then add 1ml fresh media. The p-dish will be your back-up if the coverslips don't grow or get contaminated. D . Allow the tissue in the centrifuge tube to settle while you prepare and sterile filter the collagenase
Subculture of Suspension Cell Lines
to go out of date. Shelf life is only 6 weeks at +4ºC once glutamine and serum is added. Avoid water baths from becoming dirty by using Sigma Clean (Prod. No. S5525 ). Don?t allow essential equipment to become out of calibration. Ensure microbiological
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