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文献和实验in the RC5-B using the SS-34 rotor. Gently decant the supernatant, add 80% ethanol, centrifuge as before, decant, and dry the DNA pellet in a vacuum oven. 15a. Resuspend the DNA in 10:0.1 TE buffer. For diatomaceous earth-based purification:
2-10 µg/200 µl Sonicate in water bath sonicator on high 15-30 secs. Suspension should turn from opaque to translucent. Dry suspension in the same vacuum tubes by warming them in a 45ºC water bath while evacuating using tube connected to the vacuum
should turn from opaque to translucent. Dry suspension in the same vacuum tubes by warming them in a 45ºC water bath while evacuating using tube connected to the vacuum pump via a CuSO4 drying tube. The drying process takes 2-3 minutes.
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