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文献和实验Procedures for In Vitro Production of Bovine Embryos
(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15
a chilled, sterile pipette tip to transfer the competent cells to chilled, sterile 17 x 100-mm polypropylene tubes. Store the cells on ice. Glass tubes should not be used since they lower the efficiency of transformation by ~10-fold
In Situ HYBRIDIZATION TO TISSUE SECTIONS
. The devittellinized embryos will sink to the bottom. Transfer the embryos to a vial with a wide bore pipet tip. Wash 3X with 90% MeOH/EGTA. Rehydrate embryos (0-2 and 1-3 hr embryos need not be rehydrated through paraformaldehyde, just leave
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