赛默飞世尔Thermo Fisher FUNNEL BUCH

A quick RNA mini-prep for Neurospora mycelial cultures

. A 50 ml culture typically yields enough RNA for 200 gel lanes (see below), and, as noted, smaller culture volumes may be used. 2. Flat mycelial pads are easier to grind than mycelial balls. Therefore, filter cultures using a Buchner funnel

A quick RNA mini-prep for Neurospora mycelial cultures

for 200 gel lanes (see below), and, as noted, smaller culture volumes may be used. 2. Flat mycelial pads are easier to grind than mycelial balls. Therefore, filter cultures using a Buchner funnel onto Whatman #44 filter paper. Wrap flat mycelial pads

Synthesis and Properties of 7‐Substituted 7‐Deazapurine (Pyrrolo[2,3‐d]pyrimidine) 2′‐Deoxyribonucleosides

10‐, 50‐, and 100‐mL round‐bottom flasks 3‐cm‐diameter Buchner funnel with filter paper circles Rotary